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Organotypischen Kultur der GFP-exprimierenden Maus Embryonen für Real-time Imaging der peripheren Nerven Outgrowth
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March 29, 2011
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Wir präsentieren eine Methode zur organotypischen Schnitten der Mitte der Schwangerschaft Maus-Embryonen für die Kultivierung und Zeitraffer-Bildgebung von peripheren Nerven Auswuchs vorzubereiten.
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Organotypic Slice CultureGFP-expressing Mouse EmbryosReal-time ImagingPeripheral Nerve OutgrowthEx Vivo CultivationTransgenic Mouse LineTauGFPGreen Fluorescent ProteinNeuronsCentral Nervous SystemPeripheral Nervous SystemForelimb InnervationPharmacological TechniquesGenetic TechniquesVibratomeSlice PreparationViabilityAge-specific DevelopmentSpinal NervesSpinal CordDorsal Root GangliaEmbryonic Day (E) E10 To E12 Slices
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Brachmann, I., Tucker, K. L. Organotypic Slice Culture of GFP-expressing Mouse Embryos for Real-time Imaging of Peripheral Nerve Outgrowth. J. Vis. Exp. (49), e2309, doi:10.3791/2309 (2011).
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