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Organotypic Slice Culture of GFP-expressing Mouse Embryos for Real-time Imaging of Peripheral Nerve Outgrowth
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JoVE Journal Nörobilim
Organotypic Slice Culture of GFP-expressing Mouse Embryos for Real-time Imaging of Peripheral Nerve Outgrowth

Organotypic Slice Culture of GFP-expressing Mouse Embryos for Real-time Imaging of Peripheral Nerve Outgrowth

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05:42 min

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March 29, 2011

DOI:

10.3791/2309-v

DOI:
10.3791/2309-v

•

05:42 min
March 29, 2011

•

1 Views
,
1Interdisciplinary Center for Neurosciences, Institute of Anatomy and Cell Biology,University of Heidelberg

Özet

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We present a method to prepare organotypic slices of mid-gestation mouse embryos for the cultivation and time-lapse imaging of peripheral nerve outgrowth.

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Brachmann, I., Tucker, K. L. Organotypic Slice Culture of GFP-expressing Mouse Embryos for Real-time Imaging of Peripheral Nerve Outgrowth. J. Vis. Exp. (49), e2309, doi:10.3791/2309 (2011).

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