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Organotypic Slice Culture of GFP-expressing Mouse Embryos for Real-time Imaging of Peripheral Nerve Outgrowth
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Organotypic Slice Culture of GFP-expressing Mouse Embryos for Real-time Imaging of Peripheral Nerve Outgrowth
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March 29, 2011
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Summary
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We present a method to prepare organotypic slices of mid-gestation mouse embryos for the cultivation and time-lapse imaging of peripheral nerve outgrowth.
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Organotypic Slice CultureGFP-expressing Mouse EmbryosReal-time ImagingPeripheral Nerve OutgrowthEx Vivo CultivationTransgenic Mouse LineTauGFPGreen Fluorescent ProteinNeuronsCentral Nervous SystemPeripheral Nervous SystemForelimb InnervationPharmacological TechniquesGenetic TechniquesVibratomeSlice PreparationViabilityAge-specific DevelopmentSpinal NervesSpinal CordDorsal Root GangliaEmbryonic Day (E) E10 To E12 Slices
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Brachmann, I., Tucker, K. L. Organotypic Slice Culture of GFP-expressing Mouse Embryos for Real-time Imaging of Peripheral Nerve Outgrowth. J. Vis. Exp. (49), e2309, doi:10.3791/2309 (2011).
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