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Mediada por adenovirus eliminación de moléculas de señalización genética en cultivos de fibroblastos de embriones de ratón primaria
JoVE Journal
Biology
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JoVE Journal Biology
Adenovirus-mediated Genetic Removal of Signaling Molecules in Cultured Primary Mouse Embryonic Fibroblasts
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Mediada por adenovirus eliminación de moléculas de señalización genética en cultivos de fibroblastos de embriones de ratón primaria

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24,623 Views

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11:00 min

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September 09, 2010

DOI:

10.3791/2160-v

DOI:
10.3791/2160-v

•

11:00 min
September 09, 2010

•

2 Views
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1Department of Molecular and Cellular Biology,University of Guelph

Summary

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En este vídeo se utiliza un adenovirus que porta el gen de la recombinasa Cre para infectar fibroblastos primarios de embriones de ratón que lleva un alelo floxed Rac1.

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Adenovirus-mediatedGenetic RemovalSignaling MoleculesCultured Primary Mouse Embryonic FibroblastsCre-loxP SystemConditional DeletionCre RecombinaseFloxed DNARecombination EventExcisionAdenovirusCre Recombinase GeneInfectPrimary Mouse Embryonic Fibroblasts (MEFs)Floxed Rac1 AlleleMorphological ChangesActin CytoskeletonViral TransductionCre ExpressionStained Using Phalloidin DyeConfocal Laser Scanning Microscopy

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Hawley, S. P., Wills, M. K. B., Jones, N. Adenovirus-mediated Genetic Removal of Signaling Molecules in Cultured Primary Mouse Embryonic Fibroblasts. J. Vis. Exp. (43), e2160, doi:10.3791/2160 (2010).

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