Journal
/
Biologie
/
Adenovirus-mediated Genetic Removal of Signaling Molecules in Cultured Primary Mouse Embryonic Fibroblasts
JoVE Journal
Biologie
Author Produced
This content is Free Access.
JoVE Journal Biologie
Adenovirus-mediated Genetic Removal of Signaling Molecules in Cultured Primary Mouse Embryonic Fibroblasts

Adenovirus-mediated Genetic Removal of Signaling Molecules in Cultured Primary Mouse Embryonic Fibroblasts

DOI:
10.3791/2160-v

•

11:00 min

•

September 09, 2010

•

, ,
1Department of Molecular and Cellular Biology,University of Guelph

Kapitel

  • 00:00Titel
  • 00:27Introduction
  • 02:59Materials & Methods
  • 09:36Conclusion

Summary

Automatische Übersetzung

Automatische Übersetzung

In this video we use an adenovirus carrying the Cre recombinase gene to infect primary mouse embryonic fibroblasts carrying a floxed Rac1 allele.

Tags

Adenovirus-mediatedGenetic RemovalSignaling MoleculesCultured Primary Mouse Embryonic FibroblastsCre-loxP SystemConditional DeletionCre RecombinaseFloxed DNARecombination EventExcisionAdenovirusCre Recombinase GeneInfectPrimary Mouse Embryonic Fibroblasts (MEFs)Floxed Rac1 AlleleMorphological ChangesActin CytoskeletonViral TransductionCre ExpressionStained Using Phalloidin DyeConfocal Laser Scanning Microscopy

Article

Embed

ZUR WIEDERGABELISTE HINZUFÜGEN

Usage Statistics

Verwandte Videos

Live Imaging of Dense-core Vesicles in Primary Cultured Hippocampal Neurons

Generation of Induced Pluripotent Stem Cells by Reprogramming Mouse Embryonic Fibroblasts with a Four Transcription Factor, Doxycycline Inducible Lentiviral Transduction System

Dissection of Hippocampal Dentate Gyrus from Adult Mouse

Assaying the Ability of Diffusible Signaling Molecules to Reorient Embryonic Spinal Commissural Axons

In vivo Imaging and Therapeutic Treatments in an Orthotopic Mouse Model of Ovarian Cancer

Rapid Fibroblast Removal from High Density Human Embryonic Stem Cell Cultures

A System for ex vivo Culturing of Embryonic Pancreas

Rapid Genetic Analysis of Epithelial-Mesenchymal Signaling During Hair Regeneration

Organelle Transport in Cultured Drosophila Cells: S2 Cell Line and Primary Neurons.

Glucose Uptake Measurement and Response to Insulin Stimulation in In Vitro Cultured Human Primary Myotubes

Read Article