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September 09, 2010
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このビデオでは、floxed Rac1の対立遺伝子を運ぶ主要なマウス胚性線維芽細胞を感染させるためにCreリコンビナーゼ遺伝子を有するアデノウイルスを使用してください。
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Adenovirus-mediatedGenetic RemovalSignaling MoleculesCultured Primary Mouse Embryonic FibroblastsCre-loxP SystemConditional DeletionCre RecombinaseFloxed DNARecombination EventExcisionAdenovirusCre Recombinase GeneInfectPrimary Mouse Embryonic Fibroblasts (MEFs)Floxed Rac1 AlleleMorphological ChangesActin CytoskeletonViral TransductionCre ExpressionStained Using Phalloidin DyeConfocal Laser Scanning Microscopy
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Hawley, S. P., Wills, M. K. B., Jones, N. Adenovirus-mediated Genetic Removal of Signaling Molecules in Cultured Primary Mouse Embryonic Fibroblasts. J. Vis. Exp. (43), e2160, doi:10.3791/2160 (2010).
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