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November 17, 2010
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Nous présentons ici l'injection intracrânienne de vecteurs AAV pour le marquage fluorescent des neurones et cellules gliales dans le cortex visuel.
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Intracranial InjectionAdeno-associated Viral VectorsLabeling TechniqueVisualizationSpecific Subsets Of CellsBrain RegionsIn VivoBrain SectionsFluorescent ProteinViral LabelingIndividual Cell TypesTransgenic Mouse LinesAAV VectorStereotaxic CoordinatesMicropipetteAutomated PumpPrecise DeliveryMinimal DamageInjection ParametersAnimal Age At InjectionInjection LocationVolume Of InjectionRate Of InjectionAAV SerotypePromoter Driving Gene ExpressionVirally-induced Transgene ExpressionVisualization Of Cells And ProcessesDendritic Spines
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Cite this Article
Lowery, R. L., Majewska, A. K. Intracranial Injection of Adeno-associated Viral Vectors. J. Vis. Exp. (45), e2140, doi:10.3791/2140 (2010).
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