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Intracranial Injection of Adeno-associated Viral Vectors
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Neurociência
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JoVE Journal Neurociência
Intracranial Injection of Adeno-associated Viral Vectors

Intracranial Injection of Adeno-associated Viral Vectors

DOI:
10.3791/2140-v

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08:47 min

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November 17, 2010

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1Neurobiology and Anatomy,University of Rochester

Capítulos

  • 00:00Título
  • 00:48Introduction
  • 01:03Virus Handling and Storage
  • 02:45Surgery
  • 04:50Injection Preparation
  • 05:44Virus Injection
  • 07:00Cleanup
  • 07:42Images of Virally-transduced Neurons
  • 08:19Conclusion

Summary

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Here we present the intracranial injection of AAV vectors for fluorescent labeling of neurons and glia in the visual cortex.

Tags

Intracranial InjectionAdeno-associated Viral VectorsLabeling TechniqueVisualizationSpecific Subsets Of CellsBrain RegionsIn VivoBrain SectionsFluorescent ProteinViral LabelingIndividual Cell TypesTransgenic Mouse LinesAAV VectorStereotaxic CoordinatesMicropipetteAutomated PumpPrecise DeliveryMinimal DamageInjection ParametersAnimal Age At InjectionInjection LocationVolume Of InjectionRate Of InjectionAAV SerotypePromoter Driving Gene ExpressionVirally-induced Transgene ExpressionVisualization Of Cells And ProcessesDendritic Spines

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