Rivista
/
Neuroscienze
/
Intracranial Injection of Adeno-associated Viral Vectors
JoVE Journal
Neuroscienze
È necessario avere un abbonamento a JoVE per visualizzare questo.  Accedi o inizia la tua prova gratuita.
JoVE Journal Neuroscienze
Intracranial Injection of Adeno-associated Viral Vectors

Intracranial Injection of Adeno-associated Viral Vectors

DOI:
10.3791/2140-v

•

08:47 min

•

November 17, 2010

•

,
1Neurobiology and Anatomy,University of Rochester

Capitoli

  • 00:00Titolo
  • 00:48Introduction
  • 01:03Virus Handling and Storage
  • 02:45Surgery
  • 04:50Injection Preparation
  • 05:44Virus Injection
  • 07:00Cleanup
  • 07:42Images of Virally-transduced Neurons
  • 08:19Conclusion

Summary

Traduzione automatica

Traduzione automatica

Here we present the intracranial injection of AAV vectors for fluorescent labeling of neurons and glia in the visual cortex.

Tags

Intracranial InjectionAdeno-associated Viral VectorsLabeling TechniqueVisualizationSpecific Subsets Of CellsBrain RegionsIn VivoBrain SectionsFluorescent ProteinViral LabelingIndividual Cell TypesTransgenic Mouse LinesAAV VectorStereotaxic CoordinatesMicropipetteAutomated PumpPrecise DeliveryMinimal DamageInjection ParametersAnimal Age At InjectionInjection LocationVolume Of InjectionRate Of InjectionAAV SerotypePromoter Driving Gene ExpressionVirally-induced Transgene ExpressionVisualization Of Cells And ProcessesDendritic Spines

Article

Embed

AGGIUNGI ALLA PLAYLIST

Usage Statistics

Video correlati

Targeting of Deep Brain Structures with Microinjections for Delivery of Drugs, Viral Vectors, or Cell Transplants

Limbal Approach-Subretinal Injection of Viral Vectors for Gene Therapy in Mice Retinal Pigment Epithelium

Intracerebroventricular and Intravascular Injection of Viral Particles and Fluorescent Microbeads into the Neonatal Brain

Subpial Adeno-associated Virus 9 (AAV9) Vector Delivery in Adult Mice

Methods and Tips for Intravenous Administration of Adeno-associated Virus to Rats and Evaluation of Central Nervous System Transduction

Adeno-associated Virus-mediated Transgene Expression in Genetically Defined Neurons of the Spinal Cord

Direct Intrathecal Injection of Recombinant Adeno-associated Viruses in Adult Mice

Production, Purification, and Quality Control for Adeno-associated Virus-based Vectors

Convection Enhanced Delivery of Optogenetic Adeno-associated Viral Vector to the Cortex of Rhesus Macaque Under Guidance of Online MRI Images

In Vivo Direct Reprogramming of Resident Glial Cells into Interneurons by Intracerebral Injection of Viral Vectors

Read Article