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Оценка целостности эпителиального барьера двумерных монослоев, полученных из органоидов, с помощью анализа парацеллюлярной проницаемости
JoVE Journal
Medicine
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JoVE Journal Medicine
Assessing Epithelial Barrier Integrity of Organoid-Derived 2D Monolayers Using Paracellular Permeability Assay
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Оценка целостности эпителиального барьера двумерных монослоев, полученных из органоидов, с помощью анализа парацеллюлярной проницаемости

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01:36 min

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June 14, 2024

DOI:

10.3791/201856-v

DOI:
10.3791/201856-v

•

01:36 min
June 14, 2024

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1Department of Veterinary Microbiology and Pathology, College of Veterinary Medicine,Washington State University, 2Department of Veterinary Clinical Sciences, College of Veterinary Medicine,Washington State University

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Epithelial Barrier IntegrityOrganoid-derived 2D MonolayersParacellular Permeability AssayFITC-dextran TracerFluorescence IntensityMicroplate Reader

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Dykstra, G. D., Kawasaki, M., Ambrosini, Y. M. Assessing Epithelial Barrier Integrity of Organoid-Derived 2D Monolayers Using Paracellular Permeability Assay. J. Vis. Exp. (Pending Publication), e201856, doi:10.3791/201856 (2024).

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