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Journal
/
Medizin
/
Developing 2D Monolayer Cultures from Bovine Intestinal Organoids
/
Assessing Epithelial Barrier Integrity of Organoid-Derived 2D Monolayers Using Paracellular Permeability Assay
JoVE Journal
Medizin
This content is Free Access.
JoVE Journal
Medizin
Assessing Epithelial Barrier Integrity of Organoid-Derived 2D Monolayers Using Paracellular Permeability Assay
Assessing Epithelial Barrier Integrity of Organoid-Derived 2D Monolayers Using Paracellular Permeability Assay
Developing 2D Monolayer Cultures from Bovine Intestinal Organoids
DOI:
10.3791/201856-v
•
01:36 min
•
June 14, 2024
•
Gerald D. Dykstra*
1
,
Minae Kawasaki*
2
,
Yoko M. Ambrosini
1
Department of Veterinary Microbiology and Pathology, College of Veterinary Medicine
,
Washington State University
,
2
Department of Veterinary Clinical Sciences, College of Veterinary Medicine
,
Washington State University
Tags
Epithelial Barrier Integrity
Organoid-derived 2D Monolayers
Paracellular Permeability Assay
FITC-dextran Tracer
Fluorescence Intensity
Microplate Reader
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Author Spotlight: Development and Characterization of 2D Intestinal Monolayer Models from Bovine Organoids for Pathogen Interaction Studies
Using Bovine Ileal and/or Rectal Organoids for Establishing 2D Monolayer Culture
Assessing Epithelial Barrier Integrity of Organoid-Derived 2D Monolayers Using Paracellular Permeability Assay
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