JoVE
JoVE
Faculty Resource Center
Research
Behavior
Biochemistry
Biology
Bioengineering
Cancer Research
Chemistry
Developmental Biology
Engineering
Environment
Genetics
Immunology and Infection
Medicine
Neuroscience
JoVE Journal
JoVE Encyclopedia of Experiments
JoVE Chrome Extension
Education
Biology
Chemistry
Clinical
Engineering
Environmental Sciences
Pharmacology
Physics
Psychology
Statistics
JoVE Core
JoVE Science Education
JoVE Lab Manual
JoVE Quiz
JoVE Business
Videos Mapped to your Course
Authors
Librarians
High Schools
About
Sign-In
Sign In
Contact Us
Research
JoVE Journal
JoVE Encyclopedia of Experiments
Education
JoVE Core
JoVE Science Education
JoVE Lab Manual
High Schools
EN
EN - English
CN - 中文
DE - Deutsch
ES - Español
KR - 한국어
IT - Italiano
FR - Français
PT - Português
EN
EN - English
CN - 中文
DE - Deutsch
ES - Español
KR - 한국어
IT - Italiano
FR - Français
PT - Português
Close
Research
Behavior
Biochemistry
Bioengineering
Biology
Cancer Research
Chemistry
Developmental Biology
Engineering
Environment
Genetics
Immunology and Infection
Medicine
Neuroscience
Products
JoVE Journal
JoVE Encyclopedia of Experiments
Education
Biology
Chemistry
Clinical
Engineering
Environmental Sciences
Pharmacology
Physics
Psychology
Statistics
Products
JoVE Core
JoVE Science Education
JoVE Lab Manual
JoVE Quiz
JoVE Business
Videos Mapped to Your Course
Teacher Resources
Get in Touch
Instant Trial
Log In
EN
EN - English
CN - 中文
DE - Deutsch
ES - Español
KR - 한국어
IT - Italiano
FR - Français
PT - Português
Journal
/
Medicine
/
Developing 2D Monolayer Cultures from Bovine Intestinal Organoids
/
Assessing Epithelial Barrier Integrity of Organoid-Derived 2D Monolayers Using Paracellular Permeability Assay
JoVE Journal
Medicine
This content is Free Access.
JoVE Journal
Medicine
Assessing Epithelial Barrier Integrity of Organoid-Derived 2D Monolayers Using Paracellular Permeability Assay
Assessing Epithelial Barrier Integrity of Organoid-Derived 2D Monolayers Using Paracellular Permeability Assay
Developing 2D Monolayer Cultures from Bovine Intestinal Organoids
DOI:
10.3791/201856-v
•
01:36 min
•
June 14, 2024
•
Gerald D. Dykstra*
1
,
Minae Kawasaki*
2
,
Yoko M. Ambrosini
1
Department of Veterinary Microbiology and Pathology, College of Veterinary Medicine
,
Washington State University
,
2
Department of Veterinary Clinical Sciences, College of Veterinary Medicine
,
Washington State University
Tags
Epithelial Barrier Integrity
Organoid-derived 2D Monolayers
Paracellular Permeability Assay
FITC-dextran Tracer
Fluorescence Intensity
Microplate Reader
Embed
ADD TO PLAYLIST
Usage Statistics
Related Videos
Author Spotlight: Development and Characterization of 2D Intestinal Monolayer Models from Bovine Organoids for Pathogen Interaction Studies
Using Bovine Ileal and/or Rectal Organoids for Establishing 2D Monolayer Culture
Assessing Epithelial Barrier Integrity of Organoid-Derived 2D Monolayers Using Paracellular Permeability Assay
Read Article
