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Journal
/
Biochemistry
/
Optimizing FPLC for Efficient Purification of His-Tagged FEN1 Protein
/
Purification of His-Tagged FEN1 Protein Using the FPLC System and Its Analysis Using FPLC-Linked Software
JoVE Journal
Biochemistry
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JoVE Journal
Biochemistry
Purification of His-Tagged FEN1 Protein Using the FPLC System and Its Analysis Using FPLC-Linked Software
Purification of His-Tagged FEN1 Protein Using the FPLC System and Its Analysis Using FPLC-Linked Software
Optimizing FPLC for Efficient Purification of His-Tagged FEN1 Protein
DOI:
10.3791/201755-v
•
02:58 min
•
April 26, 2024
•
Madhumita Sridharan
,
Tripthi Battapadi
,
Lata Balakrishnan
1
Department of Biology
,
Indiana University Indianapolis
Tags
6X-His-tagged Protein
Affinity Purification
Fast Protein Liquid Chromatography (FPLC)
Flap Endonuclease 1 (FEN1)
Protein Expression
Protein Purification
Protein Stability
Protein Functionality
Sample Preparation
Column Packing
Flow Rate
Elution Parameters
Chromatogram Analysis
Recombinant Protein Storage
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Purification of His-Tagged FEN1 Protein Using the FPLC System and Its Analysis Using FPLC-Linked Software
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