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Optimizing FPLC for Efficient Purification of His-Tagged FEN1 Protein
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Purification of His-Tagged FEN1 Protein Using the FPLC System and Its Analysis Using FPLC-Linked Software
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Biochemie
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JoVE Journal Biochemie
Purification of His-Tagged FEN1 Protein Using the FPLC System and Its Analysis Using FPLC-Linked Software

Purification of His-Tagged FEN1 Protein Using the FPLC System and Its Analysis Using FPLC-Linked Software

Optimizing FPLC for Efficient Purification of His-Tagged FEN1 Protein

DOI:
10.3791/201755-v

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02:58 min

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April 26, 2024

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, ,
1Department of Biology,Indiana University Indianapolis

Tags

6X-His-tagged ProteinAffinity PurificationFast Protein Liquid Chromatography (FPLC)Flap Endonuclease 1 (FEN1)Protein ExpressionProtein PurificationProtein StabilityProtein FunctionalitySample PreparationColumn PackingFlow RateElution ParametersChromatogram AnalysisRecombinant Protein Storage

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