Journal
/
Bioquímica
/
Optimizing FPLC for Efficient Purification of His-Tagged FEN1 Protein
/
Purification of His-Tagged FEN1 Protein Using the FPLC System and Its Analysis Using FPLC-Linked Software
JoVE Journal
Bioquímica
É necessária uma assinatura da JoVE para visualizar este conteúdo.  Faça login ou comece sua avaliação gratuita.
JoVE Journal Bioquímica
Purification of His-Tagged FEN1 Protein Using the FPLC System and Its Analysis Using FPLC-Linked Software

Purification of His-Tagged FEN1 Protein Using the FPLC System and Its Analysis Using FPLC-Linked Software

22 Views

•

02:58 min

•

April 26, 2024

DOI:

10.3791/201755-v

DOI:
10.3791/201755-v

•

02:58 min
April 26, 2024

•

2 Views
, ,
1Department of Biology,Indiana University Indianapolis

Explore More Videos

FEN1 ProteinPurificationFPLC SystemSample LoopPump SettingsManual Load LoopElution BufferMethod SettingsColumn TypeFlow RateEquilibration VolumeSample ApplicationColumn WashGradient VolumeFraction CollectorChromatogramPeak Integration

Read Article

Cite this Article

Purification of His-Tagged FEN1 Protein Using the FPLC System and Its Analysis Using FPLC-Linked Software. J. Vis. Exp. (Pending Publication), e201755, doi:10.3791/201755 (2024).

Download .ris file

Copy

Share Video

.

Copy