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Transformación a gran escala de plásmidos UAS-cDNA/ORF a partir de un ensamblaje modular basado en CRISPR en E. coli
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May 17, 2024
DOI:
1Clinical Research Institute, The Affiliated Nanhua Hospital, Hengyang Medical School,University of South China, 2Department of Neurology, The Affiliated Nanhua Hospital, Hengyang Medical School,University of South China, 3Department of Clinical Laboratory, The Affiliated Nanhua Hospital, Hengyang Medical School,University of South China, 4Shanghai Diabetes Institute, Shanghai Key Laboratory of Diabetes Mellitus, Shanghai Clinical Center for Diabetes,Shanghai Sixth People’s Hospital Affiliated to Shanghai Jiao Tong University School of Medicine
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Large-Scale Transformation of UAS-cDNA/ORF Plasmids from CRISPR-Based Modular Assembly into E. coli . J. Vis. Exp. (Pending Publication), e201692, doi:10.3791/201692 (2024).
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