/

/

/

Large-Scale Transformation of UAS-cDNA/ORF Plasmids from CRISPR-Based Modular Assembly into E. coli

JoVE 신문
유전학

JoVE 비디오를 활용하시려면 도서관을 통한 기관 구독이 필요합니다. 전체 비디오를 보시려면 로그인하거나 무료 트라이얼을 시작하세요.

JoVE 신문 유전학

Large-Scale Transformation of UAS-cDNA/ORF Plasmids from CRISPR-Based Modular Assembly into E. coli

Large-Scale Transformation of UAS-cDNA/ORF Plasmids from CRISPR-Based Modular Assembly into E. coli

48 Views

•

01:46 min

•

May 17, 2024

DOI:

10.3791/201692-v

DOI:

10.3791/201692-v

•

01:46 min

May 17, 2024

•

2 Views

Si Xu*^1,2, Mengyu Chen*^1,2, Guang Chen^1,3, Si Luo⁴, Wen Xue^1,2, Xuelian Liu^1,2, Jiwu Wang^1,2,3, Ping Wei⁴

¹Clinical Research Institute, The Affiliated Nanhua Hospital, Hengyang Medical School,University of South China, ²Department of Neurology, The Affiliated Nanhua Hospital, Hengyang Medical School,University of South China, ³Department of Clinical Laboratory, The Affiliated Nanhua Hospital, Hengyang Medical School,University of South China, ⁴Shanghai Diabetes Institute, Shanghai Key Laboratory of Diabetes Mellitus, Shanghai Clinical Center for Diabetes,Shanghai Sixth People’s Hospital Affiliated to Shanghai Jiao Tong University School of Medicine

Explore More Videos

UAS CDNA/ORF Plasmids CRISPR-based Modular Assembly Escherichia Coli Competent E. Coli Cells Pre-chill Incubation SOC Medium LB Plates Antibiotic Resistance Gene Shaking Incubator

Read Article