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CRISPRベースのモジュラーアセンブリから大腸菌へのUAS-cDNA/ORFプラスミドの大規模形質転換

JoVE Journal
Genetics

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JoVE Journal Genetics

Large-Scale Transformation of UAS-cDNA/ORF Plasmids from CRISPR-Based Modular Assembly into E. coli

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CRISPRベースのモジュラーアセンブリから大腸菌へのUAS-cDNA/ORFプラスミドの大規模形質転換

48 Views

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01:46 min

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May 17, 2024

DOI:

10.3791/201692-v

DOI:

10.3791/201692-v

•

01:46 min

May 17, 2024

•

2 Views

Si Xu*^1,2, Mengyu Chen*^1,2, Guang Chen^1,3, Si Luo⁴, Wen Xue^1,2, Xuelian Liu^1,2, Jiwu Wang^1,2,3, Ping Wei⁴

¹Clinical Research Institute, The Affiliated Nanhua Hospital, Hengyang Medical School,University of South China, ²Department of Neurology, The Affiliated Nanhua Hospital, Hengyang Medical School,University of South China, ³Department of Clinical Laboratory, The Affiliated Nanhua Hospital, Hengyang Medical School,University of South China, ⁴Shanghai Diabetes Institute, Shanghai Key Laboratory of Diabetes Mellitus, Shanghai Clinical Center for Diabetes,Shanghai Sixth People’s Hospital Affiliated to Shanghai Jiao Tong University School of Medicine

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