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Journal
/
Biochemistry
/
CRAF-14-3-3 Interaction Analysis in Live Cells Using a BRET-Based Approach
/
Transfection of 293FT Cells with pCMV5-NanoLuc-CRAF and pCMV5-14-3-3ζ-Halo Expression Constructs
JoVE Journal
Biochemistry
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JoVE Journal
Biochemistry
Transfection of 293FT Cells with pCMV5-NanoLuc-CRAF and pCMV5-14-3-3ζ-Halo Expression Constructs
Transfection of 293FT Cells with pCMV5-NanoLuc-CRAF and pCMV5-14-3-3ζ-Halo Expression Constructs
CRAF-14-3-3 Interaction Analysis in Live Cells Using a BRET-Based Approach
DOI:
10.3791/201479-v
•
02:01 min
•
March 01, 2024
•
Russell Spencer-Smith
2,3
1
Department of Cell and Molecular Pharmacology and Experimental Therapeutics
,
Medical University of South Carolina
,
2
Hollings Cancer Center
,
Medical University of South Carolina
,
3
Laboratory of Cell and Developmental Signaling, Center for Cancer Research
,
National Cancer Institute-Frederick
Tags
Biochemistry
CRAF
RAF Kinase
RAS GTPases
Live Cell Assay
Protein-protein Interactions
KRAS-driven Cancers
Noonan Syndrome
RAF Dimerization
RAF Autoinhibition
RAF Function
Therapeutic Targeting
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