Journal
/
Bioquímica
/
CRAF-14-3-3 Interaction Analysis in Live Cells Using a BRET-Based Approach
/
Transfection of 293FT Cells with pCMV5-NanoLuc-CRAF and pCMV5-14-3-3ζ-Halo Expression Constructs
JoVE Revista
Bioquímica
Se requiere una suscripción a JoVE para ver este contenido.  Inicie sesión o comience su prueba gratuita.
JoVE Revista Bioquímica
Transfection of 293FT Cells with pCMV5-NanoLuc-CRAF and pCMV5-14-3-3ζ-Halo Expression Constructs

Transfection of 293FT Cells with pCMV5-NanoLuc-CRAF and pCMV5-14-3-3ζ-Halo Expression Constructs

24 Views

•

02:01 min

•

March 01, 2024

DOI:

10.3791/201479-v

DOI:
10.3791/201479-v

•

02:01 min
March 01, 2024

•

2 Views
1Department of Cell and Molecular Pharmacology and Experimental Therapeutics,Medical University of South Carolina, 2Hollings Cancer Center,Medical University of South Carolina, 3Laboratory of Cell and Developmental Signaling, Center for Cancer Research,National Cancer Institute-Frederick

Explore More Videos

Transfection293FT CellsPCMV5-NanoLuc-CRAFPCMV5-14-3-3-HaloTrypsin EDTADMEMCell SuspensionNanoluciferaseExpression ConstructsTransfection MediaTransfection ReagentMicrocentrifugeHalo Tagged Proteins

Read Article

Cite this Article

Transfection of 293FT Cells with pCMV5-NanoLuc-CRAF and pCMV5-14-3-3ζ-Halo Expression Constructs. J. Vis. Exp. (Pending Publication), e201479, doi:10.3791/201479 (2024).

Download .ris file

Copy

Share Video

.

Copy