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CRAF-14-3-3 Interaction Analysis in Live Cells Using a BRET-Based Approach
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Transfection of 293FT Cells with pCMV5-NanoLuc-CRAF and pCMV5-14-3-3ζ-Halo Expression Constructs
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JoVE Journal Biochimica
Transfection of 293FT Cells with pCMV5-NanoLuc-CRAF and pCMV5-14-3-3ζ-Halo Expression Constructs

Transfection of 293FT Cells with pCMV5-NanoLuc-CRAF and pCMV5-14-3-3ζ-Halo Expression Constructs

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02:01 min

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March 01, 2024

DOI:

10.3791/201479-v

DOI:
10.3791/201479-v

•

02:01 min
March 01, 2024

•

2 Views
1Department of Cell and Molecular Pharmacology and Experimental Therapeutics,Medical University of South Carolina, 2Hollings Cancer Center,Medical University of South Carolina, 3Laboratory of Cell and Developmental Signaling, Center for Cancer Research,National Cancer Institute-Frederick

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Transfection of 293FT Cells with pCMV5-NanoLuc-CRAF and pCMV5-14-3-3ζ-Halo Expression Constructs. J. Vis. Exp. (Pending Publication), e201479, doi:10.3791/201479 (2024).

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