Journal
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Immunology and Infection
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Exploring Gut Phage-Bacteria Dynamics in Murine Models Using T4 Phage
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הכנה וטיהור של ליזטים מסוג T4 פאג' למתן אוראלי בעכברים
JoVE Journal
Immunology and Infection
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JoVE Journal Immunology and Infection
Preparation and Purification of T4 Phage Lysates for Oral Administration in Mice
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הכנה וטיהור של ליזטים מסוג T4 פאג' למתן אוראלי בעכברים

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05:18 min

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January 26, 2024

DOI:

10.3791/201384-v

DOI:
10.3791/201384-v

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05:18 min
January 26, 2024

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4 Views
, , , , , , ,
1Department of Microbiology and Immunology, Life Sciences Institute,University of British Columbia, 2Department of Biology,San Diego State University, 3School of Biomedical Engineering,University of British Columbia, 4Humans and the Microbiome Program,Canadian Institute for Advanced Research

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Keyword Extraction:T4 PhageEscherichia ColiLuria-Bertani (LB) MediumPhage LysatesOral AdministrationCentrifugal FiltrationEndotoxin RemovalSaline Magnesium (SM) BufferChloroform TreatmentBacterial CultureBacterial Debris Removal

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Cite this Article

Pett, N., Hunter, M., Carranza García, N. A., Seo, J. H., Collins, S. R., Rohwer, F., Osborne, L. C., Tropini, C. Preparation and Purification of T4 Phage Lysates for Oral Administration in Mice. J. Vis. Exp. (Pending Publication), e201384, doi:10.3791/201384 (2024).

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