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Immunology and Infection

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PRÉPARATION ET PURIFICATION DE LYSATS DE PHAGES T4 pour administration orale chez la souris

JoVE Journal
Immunology and Infection

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JoVE Journal Immunology and Infection

Preparation and Purification of T4 Phage Lysates for Oral Administration in Mice

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PRÉPARATION ET PURIFICATION DE LYSATS DE PHAGES T4 pour administration orale chez la souris

61 Views

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05:18 min

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January 26, 2024

DOI:

10.3791/201384-v

DOI:

10.3791/201384-v

•

05:18 min

January 26, 2024

•

4 Views

Nicola Pett¹, Michael Hunter¹, Natalia A. Carranza García¹, Jung Hee Seo¹, Samuel R. Collins¹, Forest Rohwer², Lisa C. Osborne¹, Carolina Tropini^1,3,4

¹Department of Microbiology and Immunology, Life Sciences Institute,University of British Columbia, ²Department of Biology,San Diego State University, ³School of Biomedical Engineering,University of British Columbia, ⁴Humans and the Microbiome Program,Canadian Institute for Advanced Research

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Keyword Extraction:T4 Phage Escherichia Coli Luria-Bertani (LB) Medium Phage Lysates Oral Administration Centrifugal Filtration Endotoxin Removal Saline Magnesium (SM) Buffer Chloroform Treatment Bacterial Culture Bacterial Debris Removal

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