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Imunologia e Infecção
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Exploring Gut Phage-Bacteria Dynamics in Murine Models Using T4 Phage
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Preparation and Purification of T4 Phage Lysates for Oral Administration in Mice
JoVE Journal
Imunologia e Infecção
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JoVE Journal Imunologia e Infecção
Preparation and Purification of T4 Phage Lysates for Oral Administration in Mice

Preparation and Purification of T4 Phage Lysates for Oral Administration in Mice

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05:18 min

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January 26, 2024

DOI:

10.3791/201384-v

DOI:
10.3791/201384-v

•

05:18 min
January 26, 2024

•

4 Views
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1Department of Microbiology and Immunology, Life Sciences Institute,University of British Columbia, 2Department of Biology,San Diego State University, 3School of Biomedical Engineering,University of British Columbia, 4Humans and the Microbiome Program,Canadian Institute for Advanced Research

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Keyword Extraction:T4 PhageEscherichia ColiLuria-Bertani (LB) MediumPhage LysatesOral AdministrationCentrifugal FiltrationEndotoxin RemovalSaline Magnesium (SM) BufferChloroform TreatmentBacterial CultureBacterial Debris Removal

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Pett, N., Hunter, M., Carranza García, N. A., Seo, J. H., Collins, S. R., Rohwer, F., Osborne, L. C., Tropini, C. Preparation and Purification of T4 Phage Lysates for Oral Administration in Mice. J. Vis. Exp. (Pending Publication), e201384, doi:10.3791/201384 (2024).

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