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Strategies for Expressing and Purifying Solute Carrier Transporters
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Cloning of Solute Carriers (SLCs) into pHTBV1.1 Bacmid for High-Throughput Protein Expression (Video) | JoVE
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Biochemistry
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JoVE Journal Biochemistry
Cloning of Solute Carriers (SLCs) into pHTBV1.1 Bacmid for High-Throughput Protein Expression

Cloning of Solute Carriers (SLCs) into pHTBV1.1 Bacmid for High-Throughput Protein Expression (Video) | JoVE

Strategies for Expressing and Purifying Solute Carrier Transporters

DOI:
10.3791/200669-v

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01:12 min

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September 29, 2023

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, , , , , , , , , , , , , , , , , , , , , ,
1Centre for Medicines Discovery, Nuffield Department of Medicine,University of Oxford, 2Nuvisan ICB GmbH, 3CeMM Research Center for Molecular Medicine of the Austrian Academy of Sciences

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Keywords: SLC ClonesPHTBV1.1 BacmidProtein ExpressionCloningRecombinant EnzymeProteinase KChemically Competent E.coli Mach1Heat Shock TransformationColony PCR

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