Journal
/
Biochemistry
/
Strategies for Expressing and Purifying Solute Carrier Transporters
/
Author Spotlight: Expression and Purification of Human Solute Carrier Transporters Using Codon-Optimized Genes
JoVE Journal
Biochemistry
A subscription to JoVE is required to view this content.  Sign in or start your free trial.
JoVE Journal Biochemistry
High-Throughput Expression and Purification of Human Solute Carriers for Structural and Biochemical Studies

Author Spotlight: Expression and Purification of Human Solute Carrier Transporters Using Codon-Optimized Genes

Strategies for Expressing and Purifying Solute Carrier Transporters

DOI:
10.3791/65878-v

•

02:10 min

•

September 29, 2023

•

, , , , , , , , , , , , , , , , , , , , , ,
1Centre for Medicines Discovery, Nuffield Department of Medicine,University of Oxford, 2Nuvisan ICB GmbH, 3CeMM Research Center for Molecular Medicine of the Austrian Academy of Sciences

Tags

Solute CarriersSLCsMembrane TransportersDrug DiscoveryHigh-throughput ExpressionProtein PurificationStructural StudiesBiochemical StudiesReSOLUTEBacMam Expression SystemCryo-EM

Embed

ADD TO PLAYLIST

Usage Statistics

Related Videos

Author Spotlight: Expression and Purification of Human Solute Carrier Transporters Using Codon-Optimized Genes

Cloning of Solute Carriers (SLCs) into pHTBV1.1 Bacmid for High-Throughput Protein Expression

Purification of Human Solute Carriers (SLCs) for Developing SLC Targeting Therapies

Read Article