Journal
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生物化学
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Strategies for Expressing and Purifying Solute Carrier Transporters
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Cloning of Solute Carriers (SLCs) into pHTBV1.1 Bacmid for High-Throughput Protein Expression
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生物化学
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Cloning of Solute Carriers (SLCs) into pHTBV1.1 Bacmid for High-Throughput Protein Expression

Cloning of Solute Carriers (SLCs) into pHTBV1.1 Bacmid for High-Throughput Protein Expression

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01:12 min

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September 29, 2023

DOI:

10.3791/200669-v

DOI:
10.3791/200669-v

•

01:12 min
September 29, 2023

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1 Views
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1Centre for Medicines Discovery, Nuffield Department of Medicine,University of Oxford, 2Nuvisan ICB GmbH, 3CeMM Research Center for Molecular Medicine of the Austrian Academy of Sciences

Tags

SLC ClonesPHTBV1.1 BacmidProtein ExpressionCloningRecombinant EnzymeProteinase KChemically Competent E.coli Mach1Heat Shock TransformationColony PCR

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Cloning of Solute Carriers (SLCs) into pHTBV1.1 Bacmid for High-Throughput Protein Expression . J. Vis. Exp. (Pending Publication), e200669, doi:10.3791/200669 (2023).

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