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Chapters
- 00:00Title
- 00:39Introduction
- 02:11Plunge Freezing Thin Bacterial Cells
- 03:41High Pressure Freezing and Cryo Sectioning of Thicker Cells
- 06:43Examination of the Cells Using the Cryo Light Microscope
- 07:49Collecting Tilt Series Using the Cryo TEM
- 09:08Tomogram Reconstruction and Database
- 10:24Tomogram Segmentation
- 10:51Movies and Animation
- 11:22Representative Movies
- 12:59Conclusion
Tags
Electron CryotomographyBacterial CellsCell ShapesCell PolarityGenome SegregationCell DivisionImaging TechnologiesFluorescence Light MicroscopyX-ray CrystallographyNMR SpectroscopyUltrastructure Of CellsThree Dimensions (3D)Macromolecular ResolutionCryo Transmission Electron Microscope (cryoTEM)Low Temperature ImagingVitreous StateCryogensEthaneEthane/propane MixturesHigh-pressure FreezingCryo-sectioningTwo-dimensional Projection ImagesTomogram
