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May 06, 2010
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We illustrate here how to use electron cryotomography (ECT) to study the ultrastructure of bacterial cells in near-native states, to "macromolecular" (~4 nm) resolution.
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Electron CryotomographyBacterial CellsCell ShapesCell PolarityGenome SegregationCell DivisionImaging TechnologiesFluorescence Light MicroscopyX-ray CrystallographyNMR SpectroscopyUltrastructure Of CellsThree Dimensions (3D)Macromolecular ResolutionCryo Transmission Electron Microscope (cryoTEM)Low Temperature ImagingVitreous StateCryogensEthaneEthane/propane MixturesHigh-pressure FreezingCryo-sectioningTwo-dimensional Projection ImagesTomogram
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Chen, S., McDowall, A., Dobro, M. J., Briegel, A., Ladinsky, M., Shi, J., Tocheva, E. I., Beeby, M., Pilhofer, M., Ding, H. J., Li, Z., Gan, L., Morris, D. M., Jensen, G. J. Electron Cryotomography of Bacterial Cells. J. Vis. Exp. (39), e1943, doi:10.3791/1943 (2010).
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