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Journal
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Biology
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细菌细胞的电子Cryotomography
JoVE Journal
Biology
This content is Free Access.
JoVE Journal
Biology
Electron Cryotomography of Bacterial Cells
Please note that all translations are automatically generated.
Click here for the English version.
细菌细胞的电子Cryotomography
DOI:
10.3791/1943-v
•
14:23 min
•
May 06, 2010
•
Songye Chen
,
Alasdair McDowall
2
,
Megan J. Dobro
,
Ariane Briegel
2
,
Mark Ladinsky
2
,
Jian Shi
,
Elitza I. Tocheva
,
Morgan Beeby
2
,
Martin Pilhofer
2
,
H. Jane Ding
,
Zhuo Li
2
,
Lu Gan
,
Dylan M. Morris
,
Grant J. Jensen
2
1
Division of Biology
,
California Institute of Technology – Caltech
,
2
Howard Hughes Medical Institute
,
California Institute of Technology – Caltech
Chapters
00:00
Title
00:39
Introduction
02:11
Plunge Freezing Thin Bacterial Cells
03:41
High Pressure Freezing and Cryo Sectioning of Thicker Cells
06:43
Examination of the Cells Using the Cryo Light Microscope
07:49
Collecting Tilt Series Using the Cryo TEM
09:08
Tomogram Reconstruction and Database
10:24
Tomogram Segmentation
10:51
Movies and Animation
11:22
Representative Movies
12:59
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
我们在这里说明如何使用电子cryotomography(ECT)的研究在附近的原生状态的细菌细胞超微结构,“大分子”(〜4 nm)的决议。
Tags
Electron Cryotomography
Bacterial Cells
Cell Shapes
Cell Polarity
Genome Segregation
Cell Division
Imaging Technologies
Fluorescence Light Microscopy
X-ray Crystallography
NMR Spectroscopy
Ultrastructure Of Cells
Three Dimensions (3D)
Macromolecular Resolution
Cryo Transmission Electron Microscope (cryoTEM)
Low Temperature Imaging
Vitreous State
Cryogens
Ethane
Ethane/propane Mixtures
High-pressure Freezing
Cryo-sectioning
Two-dimensional Projection Images
Tomogram
Article
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