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Measuring the 50% Haemolytic Complement (CH50) Activity of Serum
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The classical pathway is activated by antibody and culminates in target cell lysis. The CH50 assay provides a measure of the complement activity of a serum sample. This video demonstrates the steps involved in determining the CH50 of a serum sample, the calculations and interpreting of results.
Tags
50% Haemolytic ComplementCH50 ActivityComplement SystemTarget Cell LysisPhagocytosisOpsonisationClassical Complement PathwayScreening AssaySerum Complement ComponentsSheep Red Blood CellsRabbit Anti-sheep Red Blood Cell AntibodyHaemolysisComplement Component Absence Or ReductionHaemoglobin AbsorbanceComplement Activity Analysis
