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Measuring the 50% Haemolytic Complement (CH50) Activity of Serum
Journal JoVE
Immunologie et infection
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Journal JoVE Immunologie et infection
Measuring the 50% Haemolytic Complement (CH50) Activity of Serum

Measuring the 50% Haemolytic Complement (CH50) Activity of Serum

DOI:

08:26 min

March 29, 2010

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Summary

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The classical pathway is activated by antibody and culminates in target cell lysis. The CH50 assay provides a measure of the complement activity of a serum sample. This video demonstrates the steps involved in determining the CH50 of a serum sample, the calculations and interpreting of results.

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