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12:29 min
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February 04, 2010
DOI:
1Department of Otology and Laryngology,Harvard Medical School, 2Eaton-Peabody Laboratory,Massachusetts Eye and Ear Infirmary, 3Department of Communication Sciences and Disorders,Emerson College, 4Program in Speech and Hearing Bioscience and Technology, Division of Health Science and Technology,Harvard
Summary
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Este procedimento descreve um método para o isolamento e cultura do órgão murino de Corti com ou sem o limbo espiral e neurônios do gânglio espiral. Nós também demonstrar um método para a expressão de um gene exógeno repórter no órgão de Corti explante por eletroporação.
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Cite this Article
Parker, M., Brugeaud, A., Edge, A. S. B. Primary Culture and Plasmid Electroporation of the Murine Organ of Corti. . J. Vis. Exp. (36), e1685, doi:10.3791/1685 (2010).
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