This content is Free Access.
Primary Culture and Plasmid Electroporation of the Murine Organ of Corti.
25,478 Views
•
12:29 min
•
February 04, 2010
DOI:
1Department of Otology and Laryngology,Harvard Medical School, 2Eaton-Peabody Laboratory,Massachusetts Eye and Ear Infirmary, 3Department of Communication Sciences and Disorders,Emerson College, 4Program in Speech and Hearing Bioscience and Technology, Division of Health Science and Technology,Harvard
概要
Automatically generated
This procedure describes a method for the isolation and culture of the murine organ of Corti with or without the spiral limbus and spiral ganglion neurons. We also demonstrate a method for the expression of an exogenous reporter gene in the organ of Corti explant by electroporation.
Explore More Videos
Primary CulturePlasmid ElectroporationMurine Organ Of CortiSensory EpitheliumAuditionCochleaInner EarHair CellsMechanosensory CellsInner Hair CellsOuter Hair CellsBasilar MembraneSensorineural Hearing LossAuditory ResearchOrgan ExplantGene ExpressionStereocilia MotilityCell And Molecular BiologyHair Cell RegenerationIsolation And Culture MethodNeonatal MiceTemporal Bone Isolation
Read Article
Cite this Article
Parker, M., Brugeaud, A., Edge, A. S. B. Primary Culture and Plasmid Electroporation of the Murine Organ of Corti. . J. Vis. Exp. (36), e1685, doi:10.3791/1685 (2010).
Copy