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Primary Culture and Plasmid Electroporation of the Murine Organ of Corti.
JoVE 杂志
生物学
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JoVE 杂志 生物学
Primary Culture and Plasmid Electroporation of the Murine Organ of Corti.

Primary Culture and Plasmid Electroporation of the Murine Organ of Corti.

DOI:
10.3791/1685-v

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12:29 min

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February 04, 2010

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, ,
1Department of Otology and Laryngology,Harvard Medical School, 2Eaton-Peabody Laboratory,Massachusetts Eye and Ear Infirmary, 3Department of Communication Sciences and Disorders,Emerson College, 4Program in Speech and Hearing Bioscience and Technology, Division of Health Science and Technology,Harvard

Chapters

  • 00:00Title
  • 00:05Introduction
  • 00:44Procedure Overview
  • 01:45Day 1: Coating of Glass Coverslips and Heat Sterilization Tools
  • 02:41Day 2: Isolation and Culture of the Murine Organ of Corti
  • 08:15Applications for Isolated Organ of Corti
  • 11:08Day 4: Visualization of Electroporated Cells
  • 11:37Conclusion

Summary

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This procedure describes a method for the isolation and culture of the murine organ of Corti with or without the spiral limbus and spiral ganglion neurons. We also demonstrate a method for the expression of an exogenous reporter gene in the organ of Corti explant by electroporation.

Tags

Primary CulturePlasmid ElectroporationMurine Organ Of CortiSensory EpitheliumAuditionCochleaInner EarHair CellsMechanosensory CellsInner Hair CellsOuter Hair CellsBasilar MembraneSensorineural Hearing LossAuditory ResearchOrgan ExplantGene ExpressionStereocilia MotilityCell And Molecular BiologyHair Cell RegenerationIsolation And Culture MethodNeonatal MiceTemporal Bone Isolation

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