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Preparação e Cultura de explantes Rat Lens epitelial para estudar a diferenciação terminal
JoVE Journal
Biology
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JoVE Journal Biology
Preparation and Culture of Rat Lens Epithelial Explants for Studying Terminal Differentiation

Preparação e Cultura de explantes Rat Lens epitelial para estudar a diferenciação terminal

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12,468 Views

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15:02 min

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September 22, 2009

DOI:

10.3791/1519-v

DOI:
10.3791/1519-v

•

15:02 min
September 22, 2009

•

11 Views
, ,
1Laboratory of Molecular and Developmental Biology,National Eye Institute (NEI), National Institutes of Health (NIH)

Summary

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Explantes da região central da lente de ratos epitélio diferenciar de forma síncrona quando cultivadas na presença de FGF-2. Microscopia de imunofluorescência de tais culturas pode fornece informações romance sobre a expressão gênica e eventos de sinalização associado com a diferenciação terminal.

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Cite this Article

Zelenka, P. S., Gao, C. Y., Saravanamuthu, S. S. Preparation and Culture of Rat Lens Epithelial Explants for Studying Terminal Differentiation. J. Vis. Exp. (31), e1519, doi:10.3791/1519 (2009).

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