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Preparation and Culture of Rat Lens Epithelial Explants for Studying Terminal Differentiation
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September 22, 2009
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Explants of the central region of rat lens epithelia differentiate synchronously when cultured in the presence of FGF-2. Immunofluorescence microscopy of such cultures can provides novel information about gene expression and signaling events associated with terminal differentiation.
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PreparationCultureRat Lens Epithelial ExplantsTerminal DifferentiationAnterior SurfaceOcular LensEpithelial CellsProliferationAnnular ZoneCiliary BodyMigrationPosterior ArrayLens Fiber CellsBulk Of The LensIn Vitro InductionFGF-2Neonatal RatsLens CapsuleDissecting TweezersPeripheral RegionsCentral AreaFGF-2 ConcentrationTime CourseSignalingGene ExpressionMolecular TechniquesBiochemical TechniquesPharmacological TechniquesImmunofluorescence Microscopy
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Zelenka, P. S., Gao, C. Y., Saravanamuthu, S. S. Preparation and Culture of Rat Lens Epithelial Explants for Studying Terminal Differentiation. J. Vis. Exp. (31), e1519, doi:10.3791/1519 (2009).
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