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Preparation and Culture of Rat Lens Epithelial Explants for Studying Terminal Differentiation
JoVE 杂志
生物学
This content is Free Access.
JoVE 杂志 生物学
Preparation and Culture of Rat Lens Epithelial Explants for Studying Terminal Differentiation

Preparation and Culture of Rat Lens Epithelial Explants for Studying Terminal Differentiation

DOI:
10.3791/1519-v

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15:02 min

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September 22, 2009

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, ,
1Laboratory of Molecular and Developmental Biology,National Eye Institute (NEI), National Institutes of Health (NIH)

Chapters

  • 00:00Title
  • 00:54Introduction
  • 02:09Microdissection of Explants
  • 07:16Microdissection and Culture of Central Explants
  • 09:18Harvesting the Explants for Protein Analysis
  • 10:19Harvesting the Explants for Immunofluorescence
  • 12:15Immunofluorescence and Protein Analysis Results
  • 15:54Conclusion

Summary

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自动翻译

Explants of the central region of rat lens epithelia differentiate synchronously when cultured in the presence of FGF-2. Immunofluorescence microscopy of such cultures can provides novel information about gene expression and signaling events associated with terminal differentiation.

Tags

PreparationCultureRat Lens Epithelial ExplantsTerminal DifferentiationAnterior SurfaceOcular LensEpithelial CellsProliferationAnnular ZoneCiliary BodyMigrationPosterior ArrayLens Fiber CellsBulk Of The LensIn Vitro InductionFGF-2Neonatal RatsLens CapsuleDissecting TweezersPeripheral RegionsCentral AreaFGF-2 ConcentrationTime CourseSignalingGene ExpressionMolecular TechniquesBiochemical TechniquesPharmacological TechniquesImmunofluorescence Microscopy

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