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November 13, 2009
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De Stemgent Dox Induceerbare Mouse TF lentivirus Set kan herprogrammeren muis embryonale fibroblasten (MEF) om geïnduceerde pluripotente stamcellen (iPS) cellen. Hier hebben we het protocol voor DOX-induceerbare expressie van muis herprogrammering transcriptiefactoren Oct4 te tonen, om Sox2, Klf4 en c-Myc genereren iPS kolonies die uitdrukken gemeenschappelijke MES pluripotentie markers.
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Induced Pluripotent Stem CellsReprogrammingTranscription FactorsDoxycycline Inducible Lentiviral Transduction SystemMouse Embryonic FibroblastsPluripotencyRetrovirus-mediated DeliveryOct4Sox2C-MycKlf4Doxycycline Inducible Lentiviral Delivery SystemAdult Mouse Somatic Cell TypesEmbryonic Stem CellsRegenerative MedicineTeratoma FormationDifferentiated CellsEthical DilemmaStemgent DOX Inducible Mouse TF Lentivirus Set
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Hamilton, B., Feng, Q., Ye, M., Welstead, G. G. Generation of Induced Pluripotent Stem Cells by Reprogramming Mouse Embryonic Fibroblasts with a Four Transcription Factor, Doxycycline Inducible Lentiviral Transduction System. J. Vis. Exp. (33), e1447, doi:10.3791/1447 (2009).
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