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Generation of Induced Pluripotent Stem Cells by Reprogramming Mouse Embryonic Fibroblasts with a Four Transcription Factor, Doxycycline Inducible Lentiviral Transduction System
章
- 00:00標題
- 00:09Introduction
- 00:30Viral Transduction of Mouse Embryonic Fibroblasts
- 01:25Doxycycline Induced Reprogramming
- 03:18Immunocytochemical Analysis of Transduction Efficiency
- 05:31Isolating and Expanding Induced Pluripotent Stem Cell Colonies
- 07:39Immunocytochemical Analysis for Pluripotency
- 09:14Pluripotency Marker Analysis
- 10:31Conclusion
The Stemgent Dox Inducible Mouse TF Lentivirus Set can reprogram mouse embryonic fibroblasts (MEFs) to induced pluripotent stem (iPS) cells. Here we demonstrate the protocol for DOX-inducible expression of mouse reprogramming transcription factors Oct4, Sox2, Klf4 and c-Myc to generate iPS colonies that express common mES pluripotency markers.
タグ
Induced Pluripotent Stem CellsReprogrammingTranscription FactorsDoxycycline Inducible Lentiviral Transduction SystemMouse Embryonic FibroblastsPluripotencyRetrovirus-mediated DeliveryOct4Sox2C-MycKlf4Doxycycline Inducible Lentiviral Delivery SystemAdult Mouse Somatic Cell TypesEmbryonic Stem CellsRegenerative MedicineTeratoma FormationDifferentiated CellsEthical DilemmaStemgent DOX Inducible Mouse TF Lentivirus Set
