Dergi
/
Biyoloji
/
Generation of Induced Pluripotent Stem Cells by Reprogramming Mouse Embryonic Fibroblasts with a Four Transcription Factor, Doxycycline Inducible Lentiviral Transduction System
JoVE Journal
Biyoloji
This content is Free Access.
JoVE Journal Biyoloji
Generation of Induced Pluripotent Stem Cells by Reprogramming Mouse Embryonic Fibroblasts with a Four Transcription Factor, Doxycycline Inducible Lentiviral Transduction System

Generation of Induced Pluripotent Stem Cells by Reprogramming Mouse Embryonic Fibroblasts with a Four Transcription Factor, Doxycycline Inducible Lentiviral Transduction System

DOI:
10.3791/1447-v

•

11:48 min

•

November 13, 2009

•

, , ,
1Stemgent, 2Whitehead Institute for Biomedical Research,MIT – Massachusetts Institute of Technology

Bölümler

  • 00:00Başlık
  • 00:09Introduction
  • 00:30Viral Transduction of Mouse Embryonic Fibroblasts
  • 01:25Doxycycline Induced Reprogramming
  • 03:18Immunocytochemical Analysis of Transduction Efficiency
  • 05:31Isolating and Expanding Induced Pluripotent Stem Cell Colonies
  • 07:39Immunocytochemical Analysis for Pluripotency
  • 09:14Pluripotency Marker Analysis
  • 10:31Conclusion

Özet

Otomatik Çeviri

Otomatik Çeviri

The Stemgent Dox Inducible Mouse TF Lentivirus Set can reprogram mouse embryonic fibroblasts (MEFs) to induced pluripotent stem (iPS) cells. Here we demonstrate the protocol for DOX-inducible expression of mouse reprogramming transcription factors Oct4, Sox2, Klf4 and c-Myc to generate iPS colonies that express common mES pluripotency markers.

Etiketler

Induced Pluripotent Stem CellsReprogrammingTranscription FactorsDoxycycline Inducible Lentiviral Transduction SystemMouse Embryonic FibroblastsPluripotencyRetrovirus-mediated DeliveryOct4Sox2C-MycKlf4Doxycycline Inducible Lentiviral Delivery SystemAdult Mouse Somatic Cell TypesEmbryonic Stem CellsRegenerative MedicineTeratoma FormationDifferentiated CellsEthical DilemmaStemgent DOX Inducible Mouse TF Lentivirus Set

Article

Embed

ÇALMA LİSTESİNE EKLE

Usage Statistics

İlgili Videolar

Derivation of Human Embryonic Stem Cells by Immunosurgery

Generation of Induced Pluripotent Stem Cells by Reprogramming Human Fibroblasts with the Stemgent Human TF Lentivirus Set

Transfecting and Nucleofecting Human Induced Pluripotent Stem Cells

Efficient Derivation of Human Cardiac Precursors and Cardiomyocytes from Pluripotent Human Embryonic Stem Cells with Small Molecule Induction

Selecting and Isolating Colonies of Human Induced Pluripotent Stem Cells Reprogrammed from Adult Fibroblasts

Reprogramming Human Somatic Cells into Induced Pluripotent Stem Cells (iPSCs) Using Retroviral Vector with GFP

Preparation of Mouse Embryonic Fibroblast Cells Suitable for Culturing Human Embryonic and Induced Pluripotent Stem Cells

Generation of Mice Derived from Induced Pluripotent Stem Cells

Generation of Human Induced Pluripotent Stem Cells from Peripheral Blood Using the STEMCCA Lentiviral Vector

Generation of Human Cardiomyocytes: A Differentiation Protocol from Feeder-free Human Induced Pluripotent Stem Cells

Read Article