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Obtenção altamente purificada Toxoplasma gondii Oocistos por um gradiente de cloreto de césio descontínuos
JoVE Journal
Immunology and Infection
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JoVE Journal Immunology and Infection
Obtaining Highly Purified Toxoplasma gondii Oocysts by a Discontinuous Cesium Chloride Gradient

Obtenção altamente purificada Toxoplasma gondii Oocistos por um gradiente de cloreto de césio descontínuos

português

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13,316 Views

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11:17 min

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November 03, 2009

DOI:

10.3791/1420-v

DOI:
10.3791/1420-v

•

11:17 min
November 03, 2009

•

13 Views
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1Dynamac, Inc., 2Department of Biological Sciences,University of Cincinnati, McMicken College of Arts and Science, 3Animal Parasitic Disease Laboratory,Agricultural Research Service, U.S. Department of Agriculture, 4National Exposure Research Laboratory,US Environmental Protection Agency

Summary

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Este estudo descreve o desenvolvimento de um método modificado CsCl que facilmente purifica oocistos T. gondii a partir de fezes de gatos infectados que são adequados para biológica molecular e manipulação de cultura de tecidos

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Cite this Article

Staggs, S. E., See, M. J., Dubey, J. P., Villegas, E. N. Obtaining Highly Purified Toxoplasma gondii Oocysts by a Discontinuous Cesium Chloride Gradient. J. Vis. Exp. (33), e1420, doi:10.3791/1420 (2009).

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