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Tracking Dynamics of Muscle Engraftment in Small Animals by In Vivo Fluorescent Imaging
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Biology
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JoVE Journal Biology
Tracking Dynamics of Muscle Engraftment in Small Animals by In Vivo Fluorescent Imaging

Tracking Dynamics of Muscle Engraftment in Small Animals by In Vivo Fluorescent Imaging

DOI:
10.3791/1388-v

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08:15 min

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September 21, 2009

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, , , ,
1Department of Anesthesia,Brigham and Woman’s Hospital, 2Department of Radiology,Brigham and Woman’s Hospital

Chapters

  • 00:00Title
  • 00:11Introduction
  • 00:36Preparing GFP-Labeled Cells
  • 03:40Transplanting Cells and in vivo Fluorescence Imaging
  • 06:29In vivo Fluorescence Imaging Results
  • 07:12Conclusion

Summary

Automatic Translation

Automatic Translation

We describe an in vivo fluorescence imaging protocol to monitor muscle regeneration by GFP-labeled myoblasts after transplantation into skeletal muscles of both healthy and dystrophic mice. This protocol can be adapted to study muscle regeneration by transplantation of other types of cells and in other muscular conditions as well.

Tags

Muscle EngraftmentSmall AnimalsIn Vivo Fluorescent ImagingMuscular DystrophiesDegenerative Muscle DiseasesStem Cell BiologyCell-based TherapiesFluorescent ProteinsTransplantationNon-invasive TechniqueMuscle Engraftment EvaluationIn Vivo Fluorescence ImagingGFP-labeled CellsMouse Skeletal MusclesBackground ConcernHair Removal ReagentsCCD CameraTibialis Anterior MuscleExtensor Digitorum Longus Muscle

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