Journal
/
Biología
/
Tracking Dynamics of Muscle Engraftment in Small Animals by In Vivo Fluorescent Imaging
JoVE Revista
Biología
This content is Free Access.
JoVE Revista Biología
Tracking Dynamics of Muscle Engraftment in Small Animals by In Vivo Fluorescent Imaging

Tracking Dynamics of Muscle Engraftment in Small Animals by In Vivo Fluorescent Imaging

DOI:
10.3791/1388-v

•

08:15 min

•

September 21, 2009

•

, , , ,
1Department of Anesthesia,Brigham and Woman’s Hospital, 2Department of Radiology,Brigham and Woman’s Hospital

Capítulos

  • 00:00Título
  • 00:11Introduction
  • 00:36Preparing GFP-Labeled Cells
  • 03:40Transplanting Cells and in vivo Fluorescence Imaging
  • 06:29In vivo Fluorescence Imaging Results
  • 07:12Conclusion

Summary

Traducción Automática

Traducción Automática

We describe an in vivo fluorescence imaging protocol to monitor muscle regeneration by GFP-labeled myoblasts after transplantation into skeletal muscles of both healthy and dystrophic mice. This protocol can be adapted to study muscle regeneration by transplantation of other types of cells and in other muscular conditions as well.

Tags

Muscle EngraftmentSmall AnimalsIn Vivo Fluorescent ImagingMuscular DystrophiesDegenerative Muscle DiseasesStem Cell BiologyCell-based TherapiesFluorescent ProteinsTransplantationNon-invasive TechniqueMuscle Engraftment EvaluationIn Vivo Fluorescence ImagingGFP-labeled CellsMouse Skeletal MusclesBackground ConcernHair Removal ReagentsCCD CameraTibialis Anterior MuscleExtensor Digitorum Longus Muscle

Article

Embed

Añadir a la lista de reproducción

Usage Statistics

Videos relacionados

In vivo Micro-circulation Measurement in Skeletal Muscle by Intra-vital Microscopy

Labeling hESCs and hMSCs with Iron Oxide Nanoparticles for Non-Invasive in vivo Tracking with MR Imaging

Live Cell Imaging of F-actin Dynamics via Fluorescent Speckle Microscopy (FSM)

Evaluation of Muscle Function of the Extensor Digitorum Longus Muscle Ex vivo and Tibialis Anterior Muscle In situ in Mice

In vivo Clonal Tracking of Hematopoietic Stem and Progenitor Cells Marked by Five Fluorescent Proteins using Confocal and Multiphoton Microscopy

In Vivo 4-Dimensional Tracking of Hematopoietic Stem and Progenitor Cells in Adult Mouse Calvarial Bone Marrow

High-resolution Time-lapse Imaging and Automated Analysis of Microtubule Dynamics in Living Human Umbilical Vein Endothelial Cells

A Graphical User Interface for Software-assisted Tracking of Protein Concentration in Dynamic Cellular Protrusions

Real Time In Vivo Tracking of Thymocytes in the Anterior Chamber of the Eye by Laser Scanning Microscopy

Examination of Mitotic and Meiotic Fission Yeast Nuclear Dynamics by Fluorescence Live-cell Microscopy

Read Article