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Tracking Dynamics of Muscle Engraftment in Small Animals by In Vivo Fluorescent Imaging
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September 21, 2009
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We describe an in vivo fluorescence imaging protocol to monitor muscle regeneration by GFP-labeled myoblasts after transplantation into skeletal muscles of both healthy and dystrophic mice. This protocol can be adapted to study muscle regeneration by transplantation of other types of cells and in other muscular conditions as well.
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Muscle EngraftmentSmall AnimalsIn Vivo Fluorescent ImagingMuscular DystrophiesDegenerative Muscle DiseasesStem Cell BiologyCell-based TherapiesFluorescent ProteinsTransplantationNon-invasive TechniqueMuscle Engraftment EvaluationIn Vivo Fluorescence ImagingGFP-labeled CellsMouse Skeletal MusclesBackground ConcernHair Removal ReagentsCCD CameraTibialis Anterior MuscleExtensor Digitorum Longus Muscle
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Yang, Z., Zeng, Q., Ma, Z., Wang, Y., Xu, X. Tracking Dynamics of Muscle Engraftment in Small Animals by In Vivo Fluorescent Imaging. J. Vis. Exp. (31), e1388, doi:10.3791/1388 (2009).
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