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Efficient Vascularization of Kidney Organoids through Intracelomic Transplantation in Chicken Embryos
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Efficient Vascularization of Kidney Organoids through Intracelomic Transplantation in Chicken Embryos
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07:35 min
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February 17, 2023
DOI:
1Department of Internal Medicine – Nephrology,Leiden Üniversitesi Tıp Merkezi, 2Einthoven Laboratory of Vascular and Regenerative Medicine,Leiden Üniversitesi Tıp Merkezi, 3IBPS, CNRS UMR7622, Developmental Biology Laboratory,Sorbonne Université, 4The Novo Nordisk Foundation Center for Stem Cell Medicine (reNEW),Leiden Üniversitesi Tıp Merkezi
Özet
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Here, we present a detailed protocol for the transplantation of kidney organoids in the celomic cavity of chicken embryos. This method induces vascularization and enhanced maturation of the organoids within 8 days and can be used to study these processes in an efficient manner.
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Koning, M., Lievers, E., Jaffredo, T., van den Berg, C. W., Rabelink, T. J. Efficient Vascularization of Kidney Organoids through Intracelomic Transplantation in Chicken Embryos. J. Vis. Exp. (192), e65090, doi:10.3791/65090 (2023).
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