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Standardized Identification of Compound Structure in Tibetan Medicine Using Ion Trap Mass Spectrometry and Multiple-Stage Fragmentation Analysis
JoVE Journal
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Standardized Identification of Compound Structure in Tibetan Medicine Using Ion Trap Mass Spectrometry and Multiple-Stage Fragmentation Analysis

Standardized Identification of Compound Structure in Tibetan Medicine Using Ion Trap Mass Spectrometry and Multiple-Stage Fragmentation Analysis

DOI:
10.3791/65054-v

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09:24 min

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March 17, 2023

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, , , , , ,
1State Key Laboratory of Southwestern Chinese Medicine Resources, Innovative Institute of Chinese Medicine and Pharmacy,'Chengdu University of Traditional Chinese Medicine', 2School of Ethnic Medicine,'Chengdu University of Traditional Chinese Medicine'

'Bölümler'

  • 00:04Introduction
  • 00:51Abelmoschus manihot Seeds (AMS) Sample Preparation
  • 01:32Mass Spectrometry (MS) Setting
  • 02:12Liquid Chromatography Prerun, Method Establishment, and MS Acquisition
  • 04:07Operating Multiple-Stage Mass Spectrometry
  • 06:10Manual MSn Data Analysis
  • 06:47Results: Multiple-Stage Fragmentation and Structural Analysis of Unknown AMS Compounds
  • 08:37Conclusion

Özet

'Otomatik Çeviri'

'Otomatik Çeviri'

Here, we describe a general protocol and design that could be applied to identify trace amounts and minor constituents in the complex natural product formulations (matrixes) in Tibetan medicine.

'Etiketler'

Tibetan MedicineCompound Structure AnalysisIon Trap Mass SpectrometryMultiple-stage FragmentationAbelmoschus ManihotMetabolic IntermediatesLiquid Chromatography-mass Spectrometry (LC-MS)Sample PreparationMobile PhaseIon Source

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