Here, we describe a general protocol and design that could be applied to identify trace amounts and minor constituents in the complex natural product formulations (matrixes) in Tibetan medicine.
Fu, X., Pan, Y., Wang, Y., Pei, Z., Xu, B., Zhang, J., Su, J. Standardized Identification of Compound Structure in Tibetan Medicine Using Ion Trap Mass Spectrometry and Multiple-Stage Fragmentation Analysis. J. Vis. Exp. (193), e65054, doi:10.3791/65054 (2023).