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Standardized Identification of Compound Structure in Tibetan Medicine Using Ion Trap Mass Spectrometry and Multiple-Stage Fragmentation Analysis
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JoVE Journal Química
Standardized Identification of Compound Structure in Tibetan Medicine Using Ion Trap Mass Spectrometry and Multiple-Stage Fragmentation Analysis

Standardized Identification of Compound Structure in Tibetan Medicine Using Ion Trap Mass Spectrometry and Multiple-Stage Fragmentation Analysis

704 Views

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09:24 min

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March 17, 2023

DOI:

10.3791/65054-v

DOI:
10.3791/65054-v

•

09:24 min
March 17, 2023

•

2 Views
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1State Key Laboratory of Southwestern Chinese Medicine Resources, Innovative Institute of Chinese Medicine and Pharmacy,Chengdu University of Traditional Chinese Medicine, 2School of Ethnic Medicine,Chengdu University of Traditional Chinese Medicine

Summary

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Here, we describe a general protocol and design that could be applied to identify trace amounts and minor constituents in the complex natural product formulations (matrixes) in Tibetan medicine.

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Tibetan MedicineCompound Structure AnalysisIon Trap Mass SpectrometryMultiple-stage FragmentationAbelmoschus ManihotMetabolic IntermediatesLiquid Chromatography-mass Spectrometry (LC-MS)Sample PreparationMobile PhaseIon Source

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Fu, X., Pan, Y., Wang, Y., Pei, Z., Xu, B., Zhang, J., Su, J. Standardized Identification of Compound Structure in Tibetan Medicine Using Ion Trap Mass Spectrometry and Multiple-Stage Fragmentation Analysis. J. Vis. Exp. (193), e65054, doi:10.3791/65054 (2023).

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