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斑马鱼 Tol2 系统：一种模块化且灵活的基于网关的转基因方法
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JoVE Journal Gelişim Biyolojisi
The Zebrafish Tol2 System: A Modular and Flexible Gateway-Based Transgenesis Approach
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斑马鱼 Tol2 系统：一种模块化且灵活的基于网关的转基因方法

2,271 Views

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10:00 min

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November 30, 2022

DOI:

10.3791/64679-v

DOI:
10.3791/64679-v

•

10:00 min
November 30, 2022

•

12 Views
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1Alcohol Research Center, Department of Biochemistry and Molecular Genetics,University of Louisville

Özet

Automatically generated

这项工作描述了模块化Tol2转基因系统的协议，这是一种基于网关的克隆方法，用于创建转基因构建体并将其注入斑马鱼胚胎。

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Cite this Article

Klem, J. R., Gray, R., Lovely, C. B. The Zebrafish Tol2 System: A Modular and Flexible Gateway-Based Transgenesis Approach. J. Vis. Exp. (189), e64679, doi:10.3791/64679 (2022).

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